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Image Search Results
Journal: bioRxiv
Article Title: pH Gradient Mitigation in the Leaf Cell Secretory Pathway Alters the Defense Response of Nicotiana benthamiana to Agroinfiltration
doi: 10.1101/431767
Figure Lengend Snippet: Relative abundance of Rubisco subunits and PR proteins in non-infiltrated (n.i.), EV-infiltrated and M2-expressing leaves. (A) Relative abundance of Rubisco large (RbcL) and small (RbcS) subunits. (B) Relative abundance of PR-2 (ß-glucanase) and PR-3 (chitinase) isoforms. Data are expressed relative to EV-infiltrated leaves (arbitrary value of 1). Each bar is the mean of three biological (plant) replicate values ± se . Bars with the same letter are not significantly different (post-ANOVA LSD; P =0.05).
Article Snippet: The PR proteins were detected with rabbit polyclonal IgG directed against
Techniques: Expressing
Journal: bioRxiv
Article Title: pH Gradient Mitigation in the Leaf Cell Secretory Pathway Alters the Defense Response of Nicotiana benthamiana to Agroinfiltration
doi: 10.1101/431767
Figure Lengend Snippet: Protein secretion in the apoplast of non-infiltrated (n.i.), EV-infiltrated and M2 vector-infiltrated leaves expressing or not reporter protein pHluorin. (A) Apoplastic protein profiles following 12% w/v SDS-PAGE and Coomassie blue staining. Arrows point to the 28- and 34-kDa PR protein isoforms immunodetected above with anti-PR-2 and anti-PR-3 antibodies . Numbers on the left refer to commercial molecular weight standards (Std). (B) Abundance of the 28-kDa and 34-kDa PR proteins in apoplast protein preparations of agroinfiltrated leaves expressing (+M2) or not (−M2) the M2 channel. Abundance values were inferred from the volumic densities of Coomassie blue-stained 28-kDa and 34-kDa bands following SDS-PAGE (see panel A). Data are expressed relative to mean density values in apoplast extracts of ‘−M2’ (EV, pHluorin) infiltrated leaves. (C) Fluorescence emission at 520 nm in whole cell (Total) and apoplast protein extracts of agroinfiltrated leaves expressing pHluorin alone (−M2) or along with M2 (+M2). Data are expressed relative to fluorescence emitted in leaf extracts expressing pHluorin alone (−M2). Each bar is the mean of three biological (plant) replicates ± se .
Article Snippet: The PR proteins were detected with rabbit polyclonal IgG directed against
Techniques: Plasmid Preparation, Expressing, SDS Page, Staining, Molecular Weight, Fluorescence